abstract：:The oligosaccharide chains of pituitary glycoprotein hormones such as human thyroid-stimulating hormone (hTSH) have been shown to be important in biosynthesis, subunit association, secretion and bioactivity. However, the exact biological significance of these glycosylation variants (isoforms) remains controversial. Th...

journal_title：Molecular and cellular endocrinology

authors： Schaaf L,Leiprecht A,Saji M,Hübner U,Usadel KH,Kohn LD

更新日期：1997-09-19 00:00:00

abstract：:T3 effects on myosin heavy chain gene expression were analysed in muscles undergoing different fates during metamorphosis. Muscle fate was followed by somatic gene transfer of a constitutively expressed luciferase vector. Persistent expression was found in dorsal muscle which is remodelled during metamorphosis whilst ...

journal_title：Molecular and cellular endocrinology

authors： Sachs LM,Lebrun JJ,de Luze A,Kelly PA,Demeneix BA

更新日期：1997-08-08 00:00:00

abstract：:TGF beta1 has been detected by immunohistochemistry in the rat fetal testis. Therefore, we attempted to determine whether this factor can act as a local regulator of Leydig cell function during fetal development. An inhibitory effect of TGF beta1 on basal and luteinizing hormone (LH)-stimulated testosterone secretion ...

journal_title：Molecular and cellular endocrinology

authors： Gautier C,Levacher C,Saez JM,Habert R

更新日期：1997-07-04 00:00:00

abstract：:We previously described an androgen receptor (AR) point mutation located in the DNA-binding domain (DBD), adjacent to another AR substitution. Both were observed in two unrelated families with male breast cancer (MBC) and partial androgen insensitivity syndrome. This work was designed to determine the potential role o...

journal_title：Molecular and cellular endocrinology

authors： Poujol N,Lobaccaro JM,Chiche L,Lumbroso S,Sultan C

更新日期：1997-06-20 00:00:00

abstract：:A new analogue of recombinant human growth hormone (hGH), hGH des(1-6,14) was expressed in Escherichia coli, refolded and purified to homogeneity. The mutation decreased the hormone's ability to bind lactogenic and somatogenic receptors through its site 1, and almost completely abolished its ability to bind these rece...

journal_title：Molecular and cellular endocrinology

authors： Tchelet A,Vogel T,Helman D,Guy R,Neospouolus C,Goffin V,Djiane J,Gertler A

更新日期：1997-06-20 00:00:00

abstract：:Rats deficient in vitamin A express low levels of P4502C7 mRNA in the liver. Administration of all-trans retinoic acid (at-RA) or growth hormone (GH) to deficient animals only partially restored the expression whereas the combined treatment returned the P4502C7 mRNA levels to that observed in normal rats. That a retin...

journal_title：Molecular and cellular endocrinology

authors： Westin S,Sonneveld E,van der Leede BM,van der Saag PT,Gustafsson JA,Mode A

更新日期：1997-05-16 00:00:00

abstract：:Using a combination of in vitro assays we have evaluated whether DDT metabolites can interact with the progesterone receptor pathway in yeast expressing human progesterone receptor (hPR) and in T47D human breast cancer cells which express endogenous hPR. In transactivation assays using both yeast and T47D cells, o,p'-...

journal_title：Molecular and cellular endocrinology

authors： Klotz DM,Ladlie BL,Vonier PM,McLachlan JA,Arnold SF

更新日期：1997-04-25 00:00:00

abstract：:Glucose transport activity ([3H]D-glucose uptake) in liver sinusoidal membrane vesicles (SMVs) from hyperthyroid rats was significantly higher than that from euthyroid controls (2.1-times increase in V(max) with K(m) unchanged at approximately 18 mM), associated with increased GLUT2 expression. In contrast, glucose tr...

journal_title：Molecular and cellular endocrinology

authors： Kemp HF,Hundal HS,Taylor PM

更新日期：1997-04-04 00:00:00

abstract：:The molecular cloning and nucleotide sequencing of the gonadotropin-releasing hormone (GnRH) receptor represented an enhanced step in the experimental effort to understand this key molecule in the reproductive process at a cell and molecular level. A subsequent step in this broad effort is heterologous expression of t...

journal_title：Molecular and cellular endocrinology

authors： Neill JD,Sellers JC,Musgrove LC,Duck LW

更新日期：1997-03-28 00:00:00

abstract：:Adrenal binding protein 4 (Ad4BP) known also as steroidogenic factor 1 (SF-1) is a cell specific transcription factor regulating all steroidogenic P450 genes and is present exclusively in steroidogenic tissues. In this study, we examined whether Ad4BP expression is affected by oncogene-induced cell transformation. Usi...

journal_title：Molecular and cellular endocrinology

authors： Keren-Tal I,Dantes A,Plehn-Dujowich D,Amsterdam A

更新日期：1997-03-14 00:00:00

abstract：:We have previously shown that the tyrosine kinase receptor Flk-1 and its ligand, vascular endothelial growth factor (VEGF), may play a role in the development of fetal rat islet-like structures in vitro, possibly by stimulating the maturation of endocrine precursor cells in the pancreatic ductal epithelium. In order t...

journal_title：Molecular and cellular endocrinology

authors： Oberg-Welsh C,Sandler S,Andersson A,Welsh M

更新日期：1997-02-07 00:00:00

abstract：:Protein tyrosine kinases (PTKs) are key enzymes implicated in signal transduction pathways regulated by growth factors (GFs). We have previously shown by immunohistochemistry that the level of phosphotyrosine (pY) proteins is increased in prostatic basal epithelial cells following estrogen treatment in castrated dogs....

journal_title：Molecular and cellular endocrinology

authors： Allard P,Atfi A,Landry F,Chapdelaine A,Chevalier S

更新日期：1997-01-03 00:00:00

abstract：:Most secreted proteins are modified post-translationally with the addition of carbohydrate. It has been difficult to use crystallography to solve the structures of these proteins due to the inherent heterogeneity of the carbohydrate. The structure of the chemically deglycosylated form (hydrogen fluoride treated) of hu...

journal_title：Molecular and cellular endocrinology

authors： Lustbader JW,Pollak S,Lobel L,Trakht I,Homans S,Brown JM,Canfield RE

更新日期：1996-12-20 00:00:00

abstract：:Human chorionic gonadotropin (hCG) is synthesized primarily in the placenta while human luteinizing hormone (hLH) is produced in the pituitary. Both hormones are highly homologous in structure and both appear to be altered to analogous molecular forms as the hormones are proteolytically processed, or metabolized, from...

journal_title：Molecular and cellular endocrinology

authors： Birken S,Kovalevskaya G,O'Connor J

更新日期：1996-12-20 00:00:00

abstract：:Oncogenic osteomalacia is a condition where renal phosphate wasting occurs causing defective mineralisation, in the presence of a tumor. Cultures of cells were established from a hemangiopericytoma resected from a patient with oncogenic osteomalacia. Conditioned media from the cells inhibited phosphate uptake in oposs...

journal_title：Molecular and cellular endocrinology

authors： Nelson AE,Namkung HJ,Patava J,Wilkinson MR,Chang AC,Reddel RR,Robinson BG,Mason RS

更新日期：1996-11-29 00:00:00

abstract：:Recent work from our laboratory has focused on elucidating the mechanism of androgen regulation of the androgen receptor (AR). We have demonstrated that testosterone increases AR protein and binding within 1 h in the ventral prostate of adult rats castrated for 24 h. Cycloheximide administered with testosterone reduce...

journal_title：Molecular and cellular endocrinology

authors： Mora GR,Mahesh VB

更新日期：1996-11-29 00:00:00

abstract：:Studies on the age-related decline of growth hormone (GH) release have ignored that the population of GH-producing cells (somatotropes) is heterogeneous. In aging male rats, centrifugation of dispersed pituitary cells in a density gradient yields two somatotrope subpopulations, i.e. low- (LD) and high-density (HD) cel...

journal_title：Molecular and cellular endocrinology

authors： Dobado-Berrios PM,Ruiz-Navarro A,Lopez-Pedrera R,Gonzalez de Aguilar JL,Torronteras R,Hidalgo-Diaz C,Gracia-Navarro F

更新日期：1996-10-30 00:00:00

abstract：:The recent cloning of several cDNAs encoding prostaglandin (PG) receptors has paved the way for a more detailed investigation of the postulated regulatory role of prostaglandins in corpus luteum function. We have utilized the reverse transcription-polymerase chain reaction (RT-PCR) to isolate a mRNA encoding the ovari...

journal_title：Molecular and cellular endocrinology

authors： Olofsson JI,Leung CH,Bjurulf E,Ohno T,Selstam G,Peng C,Leung PC

更新日期：1996-10-14 00:00:00

abstract：:Using primary cultures of porcine Sertoli cells as a model, the effects of ovine prolactin (oPRL) on Sertoli cell function were investigated through FSH binding. PRL treatment (0.3-5 ng/ml) induced a dose-dependent increase (ED50 = 5.10(-11) M) of 125I-FSH binding to Sertoli cells to a maximal stimulation (about 2.5-f...

journal_title：Molecular and cellular endocrinology

authors： Guillaumot P,Tabone E,Benahmed M

更新日期：1996-09-18 00:00:00

abstract：:Insulin receptor substrate-1 (IRS-1) is a protein expressed in 3T3-L1 adipocytes that is involved in most, if not all of the biological responses to insulin. Chronic exposure of these cells to insulin down-regulates IRS-1 by stimulating its degradation (Rice, K.M., Turnbow, M.A. and Garner, C.W. (1993) Biochem. Biophy...

journal_title：Molecular and cellular endocrinology

authors： Smith LK,Rice KM,Garner CW

更新日期：1996-08-30 00:00:00

abstract：:Allatostatins with a typical YXFGL-amide C-terminus constitute a neuropeptide family, which was discovered because of its inhibitory action on insect juvenile hormone synthesis. In the search for possible new functions for allatostatins we focused our attention on the fat body. Our previous studies on the cockroach Bl...

journal_title：Molecular and cellular endocrinology

authors： Martín D,Piulachs MD,Bellés X

更新日期：1996-08-09 00:00:00

abstract：:Granulosa cell-derived inhibin A (a dimer of alpha- and beta A-subunits), activin A (a homodimer of beta A-subunits) and the activin-binding protein follistatin are important regulators of human ovarian steroidogenesis. We here studied how 8-bromo-cAMP (8br-cAMP), a protein kinase A activator, and 12-O-tetradecanoylph...

journal_title：Molecular and cellular endocrinology

authors： Tuuri T,Erämaa M,Van Schaik RH,Ritvos O

更新日期：1996-07-23 00:00:00

abstract：:The NAD+ dependent (K or type 2) isozyme of 11 beta-hydroxysteroid dehydrogenase oxidizes glucocorticoids and thus prevents them from occupying mineralocorticoid receptors. Mutations in the HSD11K (HSD11B2) gene encoding this isozyme cause a genetic form of hypertension, the syndrome of apparent mineralocorticoid exce...

journal_title：Molecular and cellular endocrinology

authors： Agarwal AK,White PC

更新日期：1996-07-23 00:00:00

abstract：:Allatostatins, peptides with an YXFGL-amide C-terminus, cause a rapid and reversible inhibition of the sesquiterpenoid juvenile hormone III biosynthesis by cockroach corpora allata in vitro. Inhibition is relieved by farnesol and by mevalonate, and neither HMG-CoA reductase nor HMG-CoA synthesis (HMG-CoA synthase and ...

journal_title：Molecular and cellular endocrinology

authors： Sutherland TD,Feyereisen R

更新日期：1996-07-01 00:00:00

abstract：:We recently cloned and sequenced two kilobases of the upstream flanking region of the mouse tissue transglutaminase gene. Transfection experiments showed that this region of the transglutaminase flanking sequence was sufficient to mediate a 4-fold induction in reporter gene expression by retinoic acid. The goal of the...

journal_title：Molecular and cellular endocrinology

authors： Yan ZH,Noonan S,Nagy L,Davies PJ,Stein JP

更新日期：1996-07-01 00:00:00

abstract：:The 11 beta-hydroxysteroid dehydrogenase type II enzyme (11 beta HSD2) protects the non-discriminating mineralocorticoid receptor from occupation by glucocorticoids. In man the enzyme is also highly expressed in the placenta where it is thought to also protect the fetus from the high circulating levels of maternal glu...

journal_title：Molecular and cellular endocrinology

authors： Li KX,Smith RE,Ferrari P,Funder JW,Krozowski ZS

更新日期：1996-06-18 00:00:00

abstract：:The transcriptional activity of various lengths of the 5'-untranslated region (UTR) of the murine LH receptor (R) gene were studied using the luciferase reporter gene in transiently transfected mouse Leydig tumor cells (mLTC-1). Chinese hamster ovarian (CHO) and HeLa cells were used as controls. The basal transcriptio...

journal_title：Molecular and cellular endocrinology

authors： El-Hefnawy T,Krawczyk Z,Nikula H,Viherä I,Huhtaniemi I

更新日期：1996-05-31 00:00:00

abstract：:The 11 beta-hydroxysteroid dehydrogenase type II enzyme (11 beta HSD2) converts cortisol into mineralocorticoid receptor inactive cortisone, thus preventing occupation of the non-selective mineralocorticoid receptor by glucocorticoids in the kidney. Mutations generating inactive enzymes have been described in the HSD1...

journal_title：Molecular and cellular endocrinology

authors： Ferrari P,Obeyesekere VR,Li K,Wilson RC,New MI,Funder JW,Krozowski ZS

更新日期：1996-05-17 00:00:00

abstract：:The gene encoding ovine 11 beta-hydroxysteroid dehydrogenase type 2 (11 beta-HSD2) was cloned and characterized. This gene consists of five exons and is greater than 4 kb in length. It contains an open reading frame of 1215 bp, which encodes a protein of 404 amino acids with a predicted MW of 44 kDa. The deduced ovine...

journal_title：Molecular and cellular endocrinology

authors： Campbell LE,Yu M,Yang K

更新日期：1996-05-17 00:00:00

abstract：:Digital video imaging indicated that about 80% of fura-2-loaded single human thyroid cells responded to TSH, resulting in an increase in intracellular Ca2+ concentration ([Ca2+]i). Most of the TSH-sensitive cells further responded to N6-(L-2-phenylisopropyl)-adenosine (PIA) showing a transient [Ca2+]i rise in a PIA do...

journal_title：Molecular and cellular endocrinology

authors： Yanagita Y,Okajima F,Sho K,Nagamachi Y,Kondo Y

更新日期：1996-04-19 00:00:00

abstract：:We have established granulosa cell lines which express constitutively the rat FSH receptors by cotransfection of primary granulosa cells obtained from preovulatory follicles with SV40 DNA, Ha-ras oncogene and a plasmid expressing FSH receptors. These cells respond specifically to ovine and human FSH by cell rounding, ...

journal_title：Molecular and cellular endocrinology

authors： Schiffer Z,Keren-Tal I,Deutsch M,Dantes A,Aharoni D,Weinerb A,Tirosh R,Amsterdam A

更新日期：1996-04-19 00:00:00

abstract：:Stimulation of insulin secretion is accompanied by changes in the phosphorylation state of several islet polypeptides. Protein (de)phosphorylation is mediated by the action of protein kinases and phosphoprotein phosphatases. In this study we have investigated expression of phospho-serine/threonine phosphatases (PPs) i...

journal_title：Molecular and cellular endocrinology

authors： Murphy LI,Jones PM

更新日期：1996-03-25 00:00:00

abstract：:Two adenosine receptors A1 and A2 are associated with either stimulation (A2) or inhibition (A1) of adenylate cyclase. Using the clonal cell line Ob1771, we have studied the expression of the two receptors during the process of adipose conversion accelerated by exposure to dexamethasone and 3-isobutyl-l-methylxanthine...

journal_title：Molecular and cellular endocrinology

authors： Børglum JD,Vassaux G,Richelsen B,Gaillard D,Darimont C,Ailhaud G,Négrel R

更新日期：1996-03-01 00:00:00

abstract：:Signaling mechanisms for Blaberus discoidalis hypertrehalosemic hormone (Bld-HrTH)-dependent glycogen phosphorylase activation were investigated in vitro using fat body of the tropical cockroach, B. discoidalis. Brief treatment of fat bodies with Bld-HrTH in the absence of extracellular Ca2+ showed a significant activ...

journal_title：Molecular and cellular endocrinology

authors： Park JH,Keeley LL

更新日期：1996-02-05 00:00:00

abstract：:Lipoprotein lipase (LPL) activity is known to be synthesized, active and functional in the 1-day-old rat liver: it peaks just at birth triggered by parturition. During suckling LPL mRNA, LPL synthesis and LPL activity are still high at 5 days and then fade reaching adult values at weaning. How LPL expression is gradua...

journal_title：Molecular and cellular endocrinology

authors： Julve J,Robert MQ,Llobera M,Peinado-Onsurbe J

更新日期：1996-01-15 00:00:00

abstract：:The placenta plays an essential role in fetal growth and the maintenance of pregnancy and its functions are strictly controlled in a stage-specific manner. To gain an insight into placental functions and their regulation, we analyzed the plasma membrane proteins of rat placenta by two-dimensional polyacrylamide gel el...

journal_title：Molecular and cellular endocrinology

authors： Ishimura R,Noda K,Hattori N,Shiota K,Ogawa T

更新日期：1995-12-29 00:00:00

abstract：:Previous studies have indicated that following nutrient ingestion, GIP is released principally from the upper small intestine. In addition to its presence in the rat small intestine, GIP transcripts have also been localized to the submandibular salivary gland (SSG). The present studies were directed to further charact...

journal_title：Molecular and cellular endocrinology

authors： Tseng CC,Boylan MO,Jarboe LA,Williams EK,Sunday ME,Wolfe MM

更新日期：1995-11-30 00:00:00

abstract：:The effects of chicken growth hormone (cGH) infusion on insulin-like growth factor (IGF-I) gene expression in rapidly-growing, meat-type chickens was investigated. Chicken GH was infused either continuously or in a pulsatile fashion to 8-week-old birds during a 7-day period. Following cGH infusion, both IGF-I peptide ...

journal_title：Molecular and cellular endocrinology

authors： Rosselot G,McMurtry JP,Vasilatos-Younken R,Czerwinski S

更新日期：1995-10-30 00:00:00

abstract：:We examined the effects of growth hormone (GH), prolactin (PRL) and insulin-like growth factor-I (IGF-I) on DNA synthesis, and insulin-like growth factor binding protein 3 (IGFBP-3) gene expression, in a rat beta-cell line INS-1. GH and PRL were equally effective in increasing DNA synthesis and IGFBP-3 gene expression...

journal_title：Molecular and cellular endocrinology

authors： De W,Bréant B,Czernichow P,Asfari M

更新日期：1995-10-30 00:00:00

abstract：:The capability of rat pituitary cells to express receptors for pituitary adenylate cyclase activating polypeptide (PACAP) and VIP was evaluated by binding studies and measurement of adenylate cyclase activity on whole gland preparations and by reverse transcriptase-polymerase chain reaction (TR-PCR) using specific pri...

journal_title：Molecular and cellular endocrinology

authors： Vertongen P,Velkeniers B,Hooghe-Peters E,Robberecht P

更新日期：1995-09-22 00:00:00